Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC480

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC480
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25f5 Donor: Jizeng, Beijing Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 111 to 148 (complement) show homology to 9b1 bases 283 to 320 (38 in longest run, maxScore = 73). (GT)5 139 to 148, (GT)6 151 to 162, (GT)12 229 to 252, Bases 114 to 148 show homology to 26e12.1 bases 41 to 75 (complement) (35 in longest run, maxScore = 76). Duplicate with 26e12.1, 25f5 has priority.
Type	PCR SSR
PCR primers	Forward cTgTcATTgTTggggTggg at 60 length 19 bases, Tm 64 Reverse gAcAccAAAggAgTAgggcg at 204 length 20 bases, Tm 64
SSR size	164 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC480
Source Species	Triticum aestivum