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GrainGenes Probe Report: WMC481

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC481
Locus	Xwmc481
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25b6 Donor: Jizeng, Beijing Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 1 to 54 (complement) show homology to 9f3.1 bases 252 to 305 (54 in longest run, maxScore = 66). (CT)13 90 to 115, (CT)16 197 to 228.
Type	PCR SSR
PCR primers	Forward AcAcAAAAAccTTgTTggTccc at 158 length 22 bases, Tm 64 Reverse gAcgAcATAgTggTgcATgAgA at 306 length 22 bases, Tm 63
SSR size	170 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC481
Source Species	Triticum aestivum