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GrainGenes Probe Report: WMC482

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC482
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25f6 Donor: Jizeng, Beijing Trimmed to insert at both ends. Bases 292 to 324 (complement) show homology to 2d8 bases 73 to 105 (33 in longest run, maxScore = 77). (CA)4 84 to 92, (CA)5 98 to 107, (CA)5 177 to 186, (GGC)5 282 to 296. Bases 98 to 195 show homology to 15c5 bases 127 to 224 (complement) (98 in longest run, maxScore = 73).
Type	PCR SSR
PCR primers	Forward ccTggccAcTAcTgggcATA at 36 length 20 bases, Tm 65 Reverse cTgccAcTgTTTgcTgcTTg at 336 length 20 bases, Tm 65
SSR size	320 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC482
Source Species	Triticum aestivum