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GrainGenes Probe Report: WMC483

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC483
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25a7 Donor: Serasem Trimmed to insert at both ends. (CA)28 290 to 345.
Type	PCR SSR
PCR primers	Forward AAAATccTAggTgTgcTcTgg at 137 length 21 bases, Tm 60 Reverse gTggAgTAgTgggTcAAAgTg at 380 length 21 bases, Tm 59
SSR size	264 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product (complex, possible dominant marker).
Sequence	WMC483
Source Species	Triticum aestivum