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GrainGenes Probe Report: WMC485

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC485
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25d7.1 Donor: Serasem Multiple inserts in this clone, this is the first insert. Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 277 to 338 (complement) show homology to 24e6 bases 230 to 291 (62 in longest run, maxScore = 105). (CA)13 341 to 366, (CA)7 381 to 394.
Type	PCR SSR
PCR primers	Forward cAgcgATggATAATTgTgTTgg at 219 length 22 bases, Tm 64 Reverse cTTggATggggATgAATTgTgT at 406 length 22 bases, Tm 65
SSR size	209 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic on acrylamide (but complex).
Sequence	WMC485
Source Species	Triticum aestivum