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GrainGenes Probe Report: WMC486

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC486  [ Marker Report ]
Locus	Xwmc486-6B Xwmc486 wmc486 wmc486-6B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25d7.2 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 213 bases located on 6B Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)28 68 to 123.
Type	PCR SSR
PCR primers	Forward ccggTAgTgggATgcATTTT at 38 length 20 bases, Tm 64 Reverse ATgcATgcTgAATccggTAA at 224 length 20 bases, Tm 63
SSR size	206 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (120-140 bases)
Sequence	WMC486
Source Species	Triticum aestivum