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GrainGenes Probe Report: WMC490

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC490
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25g8 Donor: Serasem Trimmed to insert at both ends. Bases 154 to 197 show homology to 1C2 bases 384 to 427 (44 in longest run, maxScore = 58). Forward primer is outsude this region of homology, but reverse primer matches 1c2. (GT)16 81 to 112, (CT)15 51 to 80.
Type	PCR SSR
PCR primers	Forward AcTcTTTcgAccTcATggcg at 14 length 20 bases, Tm 64 Reverse gAATAggTcgAATTgggcTcA at 162 length 21 bases, Tm 63
SSR size	169 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear (120-200 bases)
Sequence	WMC490
Source Species	Triticum aestivum