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GrainGenes Probe Report: WMC496

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC496
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e10 Donor: Serasem Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 65 to 107 (complement) show homology to 11g1 bases 73 to 115 (43 in longest run, maxScore = 35). (GA)14 66 to 93.
Type	PCR SSR
PCR primers	Forward gAcTAccgccAAAAgAcTTAcA at 5 length 22 bases, Tm 60 Reverse ccAgATcAAATcTAgAcAccgT at 192 length 22 bases, Tm 60
SSR size	209 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC496
Source Species	Triticum aestivum