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GrainGenes Probe Report: WMC507

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC507
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20g9 Donor: Byrne, Colorado State Trimmed to insert at 5' end, 3' end not located. Bases 240 to 258 (complement) show homology to 17e12 bases 296 to 314 (19 in longest run, maxScore = 38), primers outdie this region of homology. (CA)5 3 to 12, (CA)24 64 to 111.
Type	PCR SSR
PCR primers	Forward AcAccccTAgTTgcgAAgT at 10 length 19 bases, Tm 59 Reverse gcAAgTTggAcATcgAATc at 119 length 19 bases, Tm 59
SSR size	128 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC507
Source Species	Triticum aestivum