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GrainGenes Probe Report: WMC509

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC509
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20f5 Donor: Byrne, Colorado State Trimmed to insert at 3' end, 5' end not located. Bases 154 to 184 show homology to 21a10 bases 126 to 156 (31 in longest run, maxScore = 36) Primers do not match 21a10. (CA)14 152 to 179.
Type	PCR SSR
PCR primers	Forward gTAccAAgTcggcATTATccAT at 86 length 22 bases, Tm 62 Reverse ATcggTTTgTgTggAAATcTgT at 278 length 22 bases, Tm 62
SSR size	214 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC509
Source Species	Triticum aestivum