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GrainGenes Probe Report: WMC510

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC510  [ Marker Report ]
Locus	Xwmc510
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20g5 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 140 bases located on 3B, 221 bases located on 3B Trimmed to insert at 3' end, 5' end not located. Bases 275 to 296 (complement) show homology to 8h1 bases 1 to 22 (22 in longest run, maxScore = 52). Primers are outside this region of homology. (CA)4TA(CA)4 161 to 178, (CA)9 290 to 307.
Type	PCR SSR
PCR primers	Forward AcTAggggccTcgAcAAAcAT at 139 length 21 bases, Tm 64 Reverse gTTTTcgAggcccccAgTA at 336 length 19 bases, Tm 64
SSR size	216 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC510
Source Species	Triticum aestivum