Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC514

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC514
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22A1 Donor: Bright, Zeneca Trimmed to insert at both ends. Bases 134 to 158 show homology to 2g12 bases 314 to 338 (25 in longest run, maxScore = 41). (GT)7 39 to 52, (GT)5 106 to 115, (GT)12 152 to 175. May get weak fragment at 186bases due to weak internal homology to the forward primer.
Type	PCR SSR
PCR primers	Forward cATgccTATgTgATgccTgc at 2 length 20 bases, Tm 64 Reverse AccAgTgcgAgggTAgAAgc at 206 length 20 bases, Tm 64
SSR size	224 bases expected, Chinese Spring
Amplification Conditions	61C anneal, cpmplex but polymorphic on acrylamide.
Sequence	WMC514
Source Species	Triticum aestivum