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GrainGenes Probe Report: WMC518

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC518
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22b2 Donor: Bright, Zeneca No overlap between forward and reverse sequences. Trimmed to insert at 3' end, 5' end not located. Bases 79 to 96 (complement) show homology to 20b9 bases 100 to 117 (18 in longest run, maxScore = 19). NB poor quqlity sequence. (CA)24 101 to 148.
Type	PCR SSR
PCR primers	Forward AAcAATAggTTTTggggggAA at 52 length 21 bases, Tm 63 Reverse gAcccccTggTcTTgATcTTT at 167 length 21 bases, Tm 63
SSR size	136 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (70 bases). 51C anneal, product (high molecular weight).
Sequence	WMC518
Source Species	Triticum aestivum