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GrainGenes Probe Report: WMC523

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC523
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22a6 Donor: Bright, Zeneca Trimmed to insert at both ends. Bases 69 to 107 (complement) show homology to 8a12 bases 187 to 225 (39 in longest run, maxScore = 78). Reverse primer has 1 mismatch to 8a12, forward primer has no obvious match. (GT)13 147 to 172.
Type	PCR SSR
PCR primers	Forward TgATcAgATgATcgTTTATgcT at 9 length 22 bases, Tm 59 Reverse gATAATAAcAcAgAcggTTgcT at 218 length 22 bases, Tm 58
SSR size	231 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC523
Source Species	Triticum aestivum