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GrainGenes Probe Report: WMC530

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC530
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26b1.1 Donor: Madre, Secobra Multiple inserts in this clone, this is the first insert. Trimmed to insert at both ends. Bases 110 to 143 (complement) show homology to 8c1.1 bases 285 to 318 (34 in longest run, maxScore = 32) Primers show no real match to 8c1.1. (GA)15 42 to 71, (GA)5 74 to 83, (TA)5 164 to 173.
Type	PCR SSR
PCR primers	Forward cggcTcTTTAAcgAgcTgTg at 20 length 20 bases, Tm 63 Reverse cggcgcTTgTTTTcTAgggT at 198 length 20 bases, Tm 65
SSR size	198 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (160-250 bases).
Sequence	WMC530
Source Species	Triticum aestivum