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GrainGenes Probe Report: WMC531

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC531
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26b1.2 Donor: Madre, Secobra Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. Bases 168 to 202 show homology to 1C2 bases 336 to 370 (35 in longest run, maxScore = 63). Reverse primer 2 mismatches to 1c2, forward primer no match. (CT)6 137 to 146, (GT)12 147 to 170.
Type	PCR SSR
PCR primers	Forward AATgATATATcTcccTgTTcgg at 7 length 22 bases, Tm 59 Reverse TggTgATATATcgTTTgTgTcc at 198 length 22 bases, Tm 59
SSR size	213 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC531
Source Species	Triticum aestivum