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GrainGenes Probe Report: WMC533

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC533  [ Marker Report ]
Locus	Xwmc533-3D [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26h1 Donor: Madre, Secobra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 134 bases located on 3B, 152 bases located on 3D Trimmed to insert at both ends. (CA)20 189 to 228.
Type	PCR SSR
PCR primers	Forward AATTggATcggcAgTTggAg at 131 length 20 bases, Tm 64 Reverse AgcAAgcAgAgcATTgcgTT at 258 length 20 bases, Tm 65
SSR size	147 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (140-156 bases).
Sequence	WMC533
Source Species	Triticum aestivum