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GrainGenes Probe Report: WMC534

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC534
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26b2 Donor: Madre, Secobra Trimmed to insert at both ends. Bases 109 to 129 show homology to 14g2 bases 359 to 379 (21 in longest run, maxScore = 23). Primers have no apparent match to 14g2. (GT)23 82 to 127.
Type	PCR SSR
PCR primers	Forward ggAggATTTcTcTcATAcTggA at 13 length 22 bases, Tm 59 Reverse AgcgTgTTTATgATAgATgggT at 161 length 22 bases, Tm 59
SSR size	170 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (130 bases and 210 bases - dominant markers?).
Sequence	WMC534
Source Species	Triticum aestivum