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GrainGenes Probe Report: WMC540

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC540  [ Marker Report ]
Locus	Xwmc540-7B [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26f6.2 Donor: Madre, Secobra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 200 bases located on 5B, 222 bases located on 3B Multiple inserts in this clone, this is the second insert. Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 186 to 204 show homology to 8f3 bases 123 to 141 (19 in longest run, maxScore = 43). Reverse primer has 6 mismatches to 8f3, forward primer no apparent homology. (GT)9 186 to 203.
Type	PCR SSR
PCR primers	Forward cggggTccTAAcTAcggTgA at 75 length 20 bases, Tm 64 Reverse ccTgTAATggAggAcggcTg at 277 length 20 bases, Tm 65
SSR size	222 bases expected, Chinese Spring
Sequence	WMC540
Source Species	Triticum aestivum