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GrainGenes Probe Report: WMC547

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC547
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26a9 Donor: Momont Trimmed to insert at both ends. (GT)6 103 to 114, (GT)13 120 to 145.
Type	PCR SSR
PCR primers	Forward gTgcgTgcgcTTAcAcTAc at 26 length 19 bases, Tm 60 Reverse AgAAAcAcgcAgcAAgAcA at 172 length 19 bases, Tm 60
SSR size	165 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product (98-164 bases complex, possibly polymorphic).
Sequence	WMC547
Source Species	Triticum aestivum