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GrainGenes Probe Report: WMC551

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC551
Locus	Xwmc551
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26a10 Donor: Momont Trimmed to insert at 5' end, 3' end not located. Bases 181 to 224 show homology to 11g12 bases 414 to 457 (44 in longest run, maxScore = 36). Reverse primer has 6 mismatches to 11g12, forard primer no obvious match. (CA)20 124 to 163.
Type	PCR SSR
PCR primers	Forward TAcgcAAcTcAcAcgcATAcc at 21 length 21 bases, Tm 64 Reverse cTcgAggcgcgTAAcAgAATA at 173 length 21 bases, Tm 64
SSR size	173 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product. 180 bases clean.
Sequence	WMC551
Source Species	Triticum aestivum