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GrainGenes Probe Report: WMC553

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC553  [ Marker Report ]
Locus	Xwmc553-6A wmc553 wmc553-6A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26f10 Donor: Momont Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 402 to 489 show homology to 4f12 bases 162 to 249 (88 in longest run, maxScore = 114), reverse primer has 2 mismatches to 4f12, no obvious math for forward primer. (CA)24 441 to 488.
Type	PCR SSR
PCR primers	Forward cggAgcATgcAgcTAgTAA at 212 length 19 bases, Tm 60 Reverse cgccTgcAgAATTcAAcAc at 549 length 19 bases, Tm 62
SSR size	356 bases expected, Chinese Spring
Sequence	WMC553
Source Species	Triticum aestivum