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GrainGenes Probe Report: WMC554

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC554
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26c11 Donor: Momont Trimmed to insert at both ends. Bases 96 to 118 show homology to 2c4.2 bases 272 to 294 (23 in longest run, maxScore = 65). Primers have no obvious match to 2c4.2. (GT)6 9 to 20, (GT)5 65 to 75, (GT)8 97 to 112, (GT)7 233 to 246, (GT)6 305 to 316.
Type	PCR SSR
PCR primers	Forward ggATAAccgATTTgcAAcTAcAg at 42 length 23 bases, Tm 62 Reverse TTATcgAcATgcAATTgggA at 324 length 20 bases, Tm 62
SSR size	302 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (300 bases).
Sequence	WMC554
Source Species	Triticum aestivum