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GrainGenes Probe Report: WMC555

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC555
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26g11.1 Donor: Momont Multiple inserts in this clone, this is the first insert.Trimmed to insert at both ends. (GT)12 344 to 367.
Type	PCR SSR
PCR primers	Forward gAAgTcATgATTgccTTcTgcc at 88 length 22 bases, Tm 65 Reverse cgTgTTcAgAAAcAAAAgcgTg at 387 length 22 bases, Tm 65
SSR size	321 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (280-300 bases).
Sequence	WMC555
Source Species	Triticum aestivum