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GrainGenes Probe Report: WMC558

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC558
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26e12.2 Donor: Momont Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. (CA)13 101 to 126.
Type	PCR SSR
PCR primers	Forward TccgTTTggATTggATgAcT at 69 length 20 bases, Tm 62 Reverse TgggTccAAAgAAgcAgAgA at 190 length 20 bases, Tm 63
SSR size	141 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (120-140 bases).
Sequence	WMC558
Source Species	Triticum aestivum