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GrainGenes Probe Report: WMC559

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC559  [ Marker Report ]
Locus	Xwmc559-3A Sr35 wmc559
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10c3 Donor: Gale, John Innes Sequence receive 14.9.99. No (apparent) overlap between forward and reverse sequences. Trimmed to insert at 3' end, 5' end not located. (GT)16 136 to 167, (GT)14 172 to 199, (GT)10 259 to 278. Size of amplified fragment is an estimation.
Type	PCR SSR
PCR primers	Forward AcAccAcgAATgATgTgccA at 71 length 20 bases, Tm 65 Reverse AcgAcgccATgTATgcAgAA at 389 length 20 bases, Tm 64
SSR size	338 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC559
Source Species	Triticum aestivum