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GrainGenes Probe Report: WMC568

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC568
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14a10 Donor: Weining, Queensland Reverse sequence only shown. Trimmed to insert at 5' end, 3' end not located. Bases 224 to 285 (complement) show homology to 6d12 bases 169 to 230 (62 in longest run, maxScore = 126). (GA)15 305 to 334,
Type	PCR SSR
PCR primers	Forward ggAgcTcgggAAgTAgAgcAAg at 13 length 22 bases, Tm 65 Reverse cAcAcTgTcTcggccTcTcAAg at 358 length 22 bases, Tm 66
SSR size	367 bases expected, Chinese Spring
Amplification Conditions	Monomorphic at 61C anneal.
Sequence	WMC568
Source Species	Triticum aestivum