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GrainGenes Probe Report: WMC569

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC569
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14a11.1 Donor: Weining, Queensland No overlap between forward and reverse sequences. Three inserts in this clone, this is the first insert. Trimmed to insert at 3' end. Bases 9 to 66 (complement) show homology to 26b11 bases 111 to 168 (58 in longest run, maxScore = 74). (GT)36 117 to 188. Forward primer matches 26b11, apparently no match to reverse primer.
Type	PCR SSR
PCR primers	Forward AgAgTggTcgccATATAgccTTg at 50 length 23 bases, Tm 65 Reverse AccATTTccTTgATTcATccgTc at 275 length 23 bases, Tm 65
SSR size	248 bases expected, Chinese Spring
Amplification Conditions	Monomorphic 61C anneal.
Sequence	WMC569
Source Species	Triticum aestivum