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GrainGenes Probe Report: WMC570

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC570
Locus
wmc570
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 14a12
Donor: Weining, Queensland
Reverse sequence only. Bases 2 to 18 (complement) show homology to 11g5 bases 705 to 721 (17 in longest run, maxScore = 20). (GT)15 379 to 408, (GT)8 429 to 444.
Type
PCR
SSR
PCR primers
Forward ccAAgAccAATgTTcTcATcAc at 205 length 22 bases, Tm 61
Reverse TcTATcgTcccTcTTAAgccAg at 533 length 22 bases, Tm 61
SSR size
350 bases expected, Chinese Spring
Amplification Conditions
Polymorphic 61C anneal.
Sequence
WMC570
Source Species
Triticum aestivum