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GrainGenes Probe Report: WMC572

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC572
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15A1 Donor: Aventis Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 235 to 303 show homology to 12a9 bases 32 to 100 (69 in longest run, maxScore = 116). (CA)12 433 to 456, (CA)13 465 to 490, (GA)8 176 to 191. No matches between 15A1 primers and 12a9 sequence.
Type	PCR SSR
PCR primers	Forward tgcAAATgATcATgcAcgAg at 83 length 20 bases, Tm 64 Reverse acatgcTAAAcAgggTTTTcAcg at 353 length 23 bases, Tm 64
SSR size	293 bases expected, Chinese Spring
Sequence	WMC572
Source Species	Triticum aestivum