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GrainGenes Probe Report: WMC582

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC582
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15h3.3 Donor: Aventis Multiple inserts in this clone, this is the third insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 10 to 27 show homology to 24b5 bases 135 to 152 (18 in longest run, maxScore = 38). (GT)30 118 to 177. Foward primer has 7 missmatches with 24b5 sequence, reverse primer has 3 missmatches with 24b5 sequence.
Type	PCR SSR
PCR primers	Forward gAgATTcccTcTcTcgccAA at 50 length 20 bases, Tm 64 Reverse ctagcAAAgTgcgATgTAgTccc at 182 length 23 bases, Tm 64
SSR size	155 bases expected, Chinese Spring
Sequence	WMC582
Source Species	Triticum aestivum