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GrainGenes Probe Report: WMC583

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC583
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15a4 Donor: Aventis Trimmed to insert at both ends. Bases 301 to 363 show homology to 17h7 bases 245 to 307 (63 in longest run, maxScore = 157). (CA)8 102 to 117, (CA)9 128 to 145, (CT)12 78 to 101. Reverse primer has 3 missmatches with 17h7 sequence. Foward primer is unique.
Type	PCR SSR
PCR primers	Forward caTAcAATgggATTccAgcAgA at 37 length 22 bases, Tm 64 Reverse aATAAggcATTcccTcgTTcAtc at 362 length 23 bases, Tm 64
SSR size	348 bases expected, Chinese Spring
Sequence	WMC583
Source Species	Triticum aestivum