Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC585

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC585
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15f4 Donor: Aventis Trimmed to insert at both ends. Bases 141 to 167 show homology to 11e11 bases 175 to 201 (27 in longest run, maxScore = 108). (GT)11 414 to 435. Foward primer has 3 mismatches with 11e11 sequence, reverse primer has 2 missmatches with 11e11.
Type	PCR SSR
PCR primers	Forward TgcAcgggcAAAccAg at 290 length 16 bases, Tm 64 Reverse cAgTAgccAcgTcATTTccTca at 457 length 22 bases, Tm 64
SSR size	189 bases expected, Chinese Spring
Sequence	WMC585
Source Species	Triticum aestivum