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GrainGenes Probe Report: WMC588

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC588
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15f6 Donor: Aventis Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 145 to 163 (complement) show homology to 2c12 bases 227 to 245 (19 in longest run, maxScore = 77). (GT)13 75 to 100. Reverse primer has 5 mismatches to 2c12, forward primer is unique.
Type	PCR SSR
PCR primers	Forward agtgagatgTcAgAccTAccTATTTgg at 5 length 27 bases, Tm 64 Reverse gcgTgcgAcTcATcTgcTATT at 272 length 21 bases, Tm 64
SSR size	288 bases expected, Chinese Spring
Sequence	WMC588
Source Species	Triticum aestivum