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GrainGenes Probe Report: wPt-7004

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
wPt-7004
Locus
Sr28
wPt-7004
URL
Diversity Arrays Techology
Reference
ReferenceYu L et al. (2014) A consensus map for Ug99 stem rust resistance loci in wheat. Theoretical and Applied Genetics 127:1561-1581.
ReferenceRouse MN et al. (2012) Identification of markers linked to the race Ug99 effective stem rust resistance gene Sr28 in wheat (Triticum aestivum L.) Theoretical and Applied Genetics 125:877-885.
ReferenceLowe IZ and Soria MA MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr26 MAS Wheat. Marker Assisted Selection in Wheat.
Type
DArT
PCR primers
5'- CTC CCA CCA AAA CAG CCT AC -3'
5'- AGA TGC GAA TGG GCA GTT AG -3'
Amplification Conditions
Denaturing step: 94C, 7 min. Amplification step (35 cycles): 94C, 60 sec; 60C, 60 sec; 72C, 60 sec. Extension step: 72C, 5 min