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GrainGenes Probe Report: GLU Bx6

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GLU Bx6
Locus	Glu-B1
Reference	Schwarz G et al. (2004) Development and validation of a PCR-based marker assay for negative selection of the HMW glutenin allele Glu-B1-1d (Bx-6) in wheat Theoretical and Applied Genetics 109:1064-1069. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' Bx7_F: CACTGAGATGGCTAAGCGCC 3' 5' Bx7_R: GCCTTGGACGGCACCACAGG 3'
Specificity	Specific for High molecular weight (HMW) glutenin Glu-B1 Bx6 subunit. PCR primers produced a 321 bp fragment for the Bx6 genotype.