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GrainGenes Probe Report: Pina-N2

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Pina-N2
Locus	Pin-a
Reference	Chen F et al. (2012) Distribution of puroindoline alleles in bread wheat cultivars of the Yellow and Huai valley of China and discovery of a novel puroindoline a allele without PINA protein Molecular Breeding 29:371-378. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
PCR primers	5' F: TCAACATTCGTGCATCATCA 3' 5' R: CTTCATTCGTCAGAGTTCCAT 3'
Specificity	Specific to allele Pina-D1r which is null for the puroindoline Pina-D1 gene. PCR primers produce a 436 bp fragment in the presence of the Pina-D1r allele.