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GrainGenes Probe Report: BF145935

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	BF145935
Locus	Sr25 BF145935
Reference	Liu S et al. (2010) Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26 Theoretical and Applied Genetics 120:691-697. Yu LX et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr25 MAS Wheat. Marker Assisted Selection in Wheat.
Type	EST PCR
PCR primers	BF145935-F 5'- CTT CAC CTC CAA GGA GTT CCA C -3' BF145935-R 5'- GCG TAC CTG ATC ACC ACC TTG AAG G -3'
Amplification Conditions	Denaturing step: 94C, 4 min. Amplification step (35 cycles): 94C, 45 sec; 50C, 30 sec; 72C, 45 sec. Extension step: 72C, 7 min