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GrainGenes Probe Report: PSY-E1

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	PSY-E1
Locus	Sr25
Reference	Zhang W and Dubcovsky J (2008) Association between allelic variation at the Phytoene synthase 1 gene and yellow pigment content in the wheat grain. Theoretical and Applied Genetics 116:635. Yu LX et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr25 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	A combination of two gene specific markers for PSY-D1 and PSY-E1 can be used to generate a codominate marker for Sr25
PCR primers	PSY1_EF2 5'- CTA CGT TGC GGG CAC CGT T -3' PSY1_ER4 5'- AGA GAA AAC CAT TGC ATC TGT A -3'
Amplification Conditions	Denaturing step: 94C, 4 min. Touchdown step (10 cycles): 94C, 20 sec; 63C, 20 sec (decrease 0.5C every step); 72C, 1 min 20 sec. Amplification step (35 cycles): 94C, 20 sec; 58C, 20 sec; 72C, 1 min 20 sec. Extension step: 72C, 7 min. Standby at 15C