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GrainGenes Probe Report: BE518379

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	BE518379
Locus	Sr26 BE518379
Reference	Liu S et al. (2010) Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26 Theoretical and Applied Genetics 120:691-697. Lowe IZ and Soria MA MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr26 MAS Wheat. Marker Assisted Selection in Wheat.
Type	EST PCR
PCR primers	5'- AGC CGC GAA ATC TAC TTT GA -3 5'- TTA AAC GGA CAG AGC ACA CG -3'
Amplification Conditions	Denaturing step: 94C, 3 min. Amplification step (35 cycles): 94C, 60 sec; 60C, 60 sec; 72C, 120 sec. Extension step: 72C, 10 min