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GrainGenes Probe Report: Sr39-50s

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Sr39-50s
Locus	Sr39
Reference	Mago R et al. (2009) Development of wheat lines carrying stem rust resistance gene Sr39 with reduced Aegilops speltoides chromatin and simple PCR markers for marker-assisted selection Theoretical and Applied Genetics 119:1441-1450. Mago R and Dundas I MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr39 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5'- CCA ATG AGG AGA TCA AAA CAA CC -3' 5'- CTA GCA AGG ACC AAG CAA TCT TG -3'
Amplification Conditions	Denaturing step: 94C, 3 min. Touchdown step 1 (7 cycles, 1C down each step): 92C, 30 sec; 65C, 30 sec; 72C, 40 sec. Amplification step (30 cycles): 94C, 30 sec; 58C, 30 sec; 72C, 40 sec. Extension step: 72C, 10 min