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GrainGenes Probe Report: Wx-B1

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
Wx-B1
Reference
ReferenceSaito M et al. (2009) A novel codominant marker for selection of the null Wx-B1 allele in wheat breeding programs. Molecular Breeding 23:209-217.
ReferenceMAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
ReferenceMAS Wheat. Bringing Genomics to the Wheat Fields. Quality Traits. Waxy mutants MAS Wheat. Marker Assisted Selection in Wheat.
PCR primers
5' BDFL: CTGGCCTGCTACCTCAAGAGCAACT 3'
5' BRC1: GGTTGCGGTTGGGGTCGATGAC 3'
Specificity
Specific to wild type allele for the gene Wx-B1 encoding waxy protein. PCR primers produce a 778 bp fragment in the presence of the wild type Wx-B1 allele.