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GrainGenes Probe Report: Rg-B1

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Rg-B1
Locus	Rg1
Reference	Abrouk M et al. (2021) Population genomics and haplotype analysis in spelt and bread wheat identifies a gene regulating glume color Communications Biology 4.
General Remarks	Amplicon: Group 1, 2, 4, 5: 381bp. Group 3: 334bp Amplification conditions: A touch-down PCR protocol was run as follows: initial denaturation at 94 degr. C for 30 seconds; annealing at 62 degr. C for 30 seconds, decreasing by 0.5 degr. C/cycle; extension at 72 degr. C for 45 seconds, followed by repeating these steps for eight cycles. After enrichment, the program continued for 29 cycles as follows: 94 degr. C for 30 seconds, 58 degr. C for 30 seconds and 72 degr. C for 45 seconds. Group 3 is associated with the red glume gene
Type	STS
PCR primers	CTCGCAAAATACATWGCTGG ATGSTGGAGTAATCCAAGGC
Sequence	Group_1,2,4,5_allele Group_3_allele
Source Species	Triticum aestivum
Source Germplasm	Jagger