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GrainGenes Probe Report: PLATZ-A1_13bpDel

[What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	PLATZ-A1_13bpDel
Reference	Zhang J et al. (2023) Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) Proceedings of the National Academy of Sciences, USA 120. Dubcovsky J MAS Wheat. Bringing Genomics to the Wheat Fields. Plant height gene Rht25. MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	'13-bp deletion found in the third exon (CS RefSeq v1.1 6A: 145, 651, 904 - 145, 651, 916) of PLATZ-A1 in UC1110, the cultivar where the first dwarfing allele of Rht25 was found' See the MASWheat webpage for amplification conditions and note that those conditions should be considered only as a starting point of the method optimization at individual laboratories.
Type	KASP
PCR primers	Plz-13bp-wt-FAM 5'- GAAGGTGACCAAGTTCATGCTGCCGAGGCCTCAGTCCA -3' Plz-13bp-mut-VIC 5'- GAAGGTCGGAGTCAACGGATTGCCGAGGCCTCAGTCGG -3' Plz-del-common 5'- TCCGACGGGACAATTAGGC -3'