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GrainGenes Probe Report: Rht25_PLATZ_CAPS

[What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Rht25_PLATZ_CAPS
Reference	Zhang J et al. (2023) Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) Proceedings of the National Academy of Sciences, USA 120. Dubcovsky J MAS Wheat. Bringing Genomics to the Wheat Fields. Plant height gene Rht25. MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	The forward primer hybridizes in the intron region. See the MASWheat webpage for amplification conditions and note that those conditions should be considered only as a starting point of the method optimization at individual laboratories.
Type	CAPS
PCR primers	PlzAF2 5'-CTGTATATGCATGTGTGTCTC-3' PlzAR1 5'-GAGGCAGCAAAAGTGGAAGG-3'