GrainGenes Reference Report: PLC-16-2514
Reference
PLC-16-2514
Title
Interaction-dependent gene expression in Mla-specified response to barley powdery mildew
Journal
Plant Cell
Year
2004
Volume
16
Pages
2514-2528
Author
Caldo RA Nettleton D Wise RP
Abstract
Plant recognition of pathogen-derived molecules influences attack and counterattack strategies that affect the outcome of host-microbe interactions. To ascertain the global framework of host gene expression during biotrophic pathogen invasion, we analysed in parallel the mRNA abundance of 22 792 host genes throughout 36 (genotype x pathogen x time) interactions between barley (Hordeum vulgare cv. Manchuria) and Blumeria graminis f.sp. hordei (Bgh), the causal agent of powdery mildew disease. A split-split-plot design was used to investigate near-isogenic barley lines with introgressed Mla6, Mla13 and Mla1 coiled-coil, nucleotide binding site, leucine-rich repeat resistance alleles challenged with Bgh isolates 5874 (AvrMla6 and AvrMla1) and K1 (AvrMla13 and AvrMla1). A linear mixed model analysis was employed to identify genes with significant differential expression (P value <0.0001) in incompatible and compatible barley-Bgh interactions across 6 time points after pathogen challenge. Twenty-two host genes, of which 5 were of unknown function, exhibited highly similar patterns of upregulation among all incompatible and compatible interactions up to 16 h after inoculation (hai), coinciding with germination of Bgh conidiospores and formation of appressoria. By contrast, significant divergent expression was observed from 16 to 32 hai, during membrane-to-membrane contact between fungal haustoria and host epidermal cells, with notable suppression of most transcripts identified as differentially expressed in compatible interactions. These findings provide a link between the recognition of general and specific pathogen-associated molecules in gene-for-gene specified resistance and support the hypothesis that host-specific resistance evolved from the recognition and prevention of the pathogen's suppression of plant basal defence
External Databases
Pubmed: 15319481
Keyword
allele
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PLC-16-2514
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||