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GrainGenes Reference Report: PLC-59-71

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Reference
PLC-59-71
Title
Genotype variation in anther culture and effect of ovary coculture in durum wheat
Journal
Plant Cell
Year
2000
Volume
59
Pages
71-76
Author
Aiti FJ
Benlhabib O
Sharma HC
El Jaafari S
El Hadrami I
Abstract
Summary: The response of anthers to in vitro culture and the effect of coculture of ovaries on anther culturability have been studied in responsive and recalcitrant cultivars of durum wheat (Triticum turgidum ssp. durum) from Morocco and ICARDA. A large genotypic-dependence of anther culture has been shown in 18 cultivars. Their response in term of callus and embryo induction varied from 0 to 13%. Coculture of ovaries with anthers enhanced the response of the most responsive genotype (cv. Sarif) and removed the recalcitrance in Cocorit and Isly cultivars. However, there was no effect of anther-ovary coculture on green plant regeneration. The implication of the genome and the media conditioning by the ovaries on anther response is discussed
Keyword
androgenesis
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