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GrainGenes Reference Report: PMB-11-95

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Reference	PMB-11-95
Title	Assembly of the barley light-harvesting chlorophyll a-b proteins in barley etiochloroplasts involves processing of the precursor on thylakoids
Journal	Plant Molecular Biology
Year	1988
Volume	11
Pages	95-108
Author	Chitnis PR Morishige DT Nechushtai R Thornber JP
Type	Article
Language	English
Abstract	A barley gene encoding the major light-harvesting chlorophyll a/b- binding protein (LHCP) has been sequenced and then expressed in vitro to produce a labelled LHCP precursor (pLHCP). When barley etiochloroplasts are incubated with this pLHCP, both labelled pLHCP and LHCP are found as integral thylakoid membrane proteins, incoroporated into the major pigment-protein complex of the thylakoids. The presence of pLHCP in thylakoids and its proportion with respect to labelled LHCP depends on the development stage of the plastids used to study the import of pLHCP. The reduced amounts of chlorophyll in a chlorophyll b-less mutant of barley does not affect the proportion of pLHCP to LHCP found in the thylakoids when import of pLHCP into plastids isolated from the mutant plants is examined. Therefore, insufficient chlorophyll during early stages of plastid development does not seem to be responsible for their relative inefficiency in assembling pLHCP. A chase of labelled pLHCP that has been incorporated into the thylakoids of intact plastids, by further incubation of the plastids with unlabelled pLHCP, reveals that the pLHCP incorporated into the thylakoids can be processed to its mature size. Our observations strongly support the hypothesis that after import into plastids, pLHCP is inserted into thylakoids and then processed to its mature size under in vivo conditions.
Probe	pCab-2
Keyword	Gene sequencing

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