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GrainGenes Reference Report: PMB-22-153

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Reference	PMB-22-153
Title	Repeated sequences as genetic markers in pooled tissue samples
Journal	Plant Molecular Biology
Year	1993
Volume	22
Pages	153-157
Author	Hellens R Ellis T Lee D Turner L
Abstract	We show, using the PDR1 element of pea, that dispersed repeated sequences of moderate copy number can be used simply and efficiently to generate markers linked to a trait of interest Inspection of hybridization patterns of repeated sequences to DNA mixtures of pooled genotypes is a sensitive way of detecting such markers The large number of bands in tracks of digests of these mixtures allows the simultaneous sampling of loci at many places in the genome, and the many unlinked loci serve as internal controls It is also shown that intensity ratios calculated from these band differences can be used to give a rough estimate of linkage distance 0388-48560 We isolated a rice cDNA clone encoding the ubiquitin protein fused to a ribosomal protein This clone encodes a single ubiquitin polypeptide and extension protein of 53 amino acids. This extension protein shows a high degree of homology with those of the yeast ubi1 or ubi2 gene, both of which encode the same protein. Northern blot analysis suggested that the expression pattern of this gene is more similar to other ribosomal protein genes not linked to ubiquitin protein than to the polyubiquitin gene.
Keyword	dispersed-repeated-sequences [ Show all 7 ]

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