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GrainGenes Reference Report: BCG-31-133

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Reference	BCG-31-133
Title	Identification, characterization, and comparison of RNA-degrading enzymes of wheat and barley
Journal	Biochemical Genetics
Year	1993
Volume	31
Pages	133-145
Author	Yen Y Baenziger P
Abstract	RNA-degrading enzymes play an important role in regulating gene expression, and sequence analyses have revealed significant homology among several plant RNA-degrading enzymes In this study we surveyed crude extracts of the above- ground part of the common wheat (Triticum aestivum L. and the cultivated barley (Hordeum vulgare L. for major RNA-degrading enzymes using a substrate-based SDS-PAGE assay Fifteen wheat and fourteen barley, RNA-degrading enzymes, with apparent molecular masses ranging from 16.3 to 40.1 kD, were identified These RNA-degrading enzymes were characterized by their response to pH changes and addition of EDTA and ZnCl2 to the preincubation or incubation buffers. The 33.2- to 40.1-kD wheat and barley, 31.7-kD wheat. and 32.0-kD barley enzyme activities were inhibited by both zinc and EDTA and were relatively tolerant to alkaline environment. The 22.7- to 28.2-kD enzymes were inhibited by zinc but stimulated by EDTA. The 18.8-kD enzyme exists in both wheat and barley. It was active in all acid environment, was inhibited by zinc, but was not affected by EDTA. Two enzyme activities (31.0 and 32.0 kD) are unique to the common wheat.
Keyword	edta [ Show all 7 ]

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